led to the following conclusions.

Paclitaxel (10 µg/mL) Cisplatin (0.5µg/mL)

DLD1 NCI/ADR DLD1 NCI/ADR

Static Perfusion S-P Static Perfusion P-S Static Perfusion S-P Static Perfusion S-P

2D 92.76 85.75 7.01 91.78 89.27 2.51 61.21 57.56 3.65 52.56 52.07 0.49

3D Spheroid 77.40 81.06 -3.66 77.61 80.73 -3.12 54.60 49.27 5.33 48.39 44.48 3.91

3D MTs 72.15 73.59 -1.44 76.82 78.64 -1.82 51.07 41.70 9.37 45.58 31.58 14

Real tumor 68.08 62.14 5.94 62.11 60.15 1.96 40.92 32.36 8.56 31.18 25.85 5.33

—

the following table lists different toxicity effect values for given concentrations under static and perfusion culture environments. A comparative study of the differences in the toxicity of the drugs under static and perfusion conditions